Human Tau proteins ELISA kit
Product Details
| Target Name |
microtubule-associated protein tau |
| Alternative Names |
AI413597 ELISA Kit; AW045860 ELISA Kit; DDPAC ELISA Kit; FLJ31424 ELISA Kit; FTDP 17 ELISA Kit; G protein beta1/gamma2 subunit interacting factor 1 ELISA Kit; MAPT ELISA Kit; MAPTL ELISA Kit; MGC134287 ELISA Kit; MGC138549 ELISA Kit; MGC156663 ELISA Kit; Microtubule associated protein tau ELISA Kit; Microtubule associated protein tau isoform 4 ELISA Kit; Microtubule-associated protein tau ELISA Kit; MSTD ELISA Kit; Mtapt ELISA Kit; MTBT1 ELISA Kit; MTBT2 ELISA Kit; Neurofibrillary tangle protein ELISA Kit; Paired helical filament tau ELISA Kit; Paired helical filament-tau ELISA Kit; PHF tau ELISA Kit; PHF-tau ELISA Kit; PPND ELISA Kit; PPP1R103 ELISA Kit; Protein phosphatase 1, regulatory subunit 103 ELISA Kit; pTau ELISA Kit; RNPTAU ELISA Kit; TAU ELISA Kit; TAU_HUMAN ELISA Kit; Tauopathy and respiratory failure ELISA Kit; Tauopathy and respiratory failure, included ELISA Kit |
| Abbreviation |
MAPT |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cerebrospinal fluid (CSF) |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-106 |
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| 1:4 |
Average % |
98 |
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| Range % |
93-105 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human Tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
95-105 |
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| EDTA plasma (n=4) |
97 |
91-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.319 |
2.423 |
2.371 |
2.228 |
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| 500 |
1.828 |
1.864 |
1.846 |
1.703 |
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| 250 |
1.291 |
1.324 |
1.308 |
1.165 |
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| 125 |
0.776 |
0.796 |
0.786 |
0.643 |
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| 62.5 |
0.464 |
0.498 |
0.481 |
0.338 |
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| 31.2 |
0.321 |
0.341 |
0.331 |
0.188 |
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| 15.6 |
0.215 |
0.227 |
0.221 |
0.078 |
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| 0 |
0.142 |
0.144 |
0.143 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
