Human Thioredoxin domain-containing protein 5(TXNDC5) ELISA kit
Product Details
| Target Name |
thioredoxin domain containing 5 (endoplasmic reticulum) |
| Alternative Names |
EndoPDI ELISA Kit; Endoplasmic reticulum protein ERp46 ELISA Kit; Endoplasmic reticulum resident protein 46 ELISA Kit; Endothelial protein disulphide isomerase ELISA Kit; ER protein 46 ELISA Kit; ERp46 ELISA Kit; Hcc 2 ELISA Kit; MGC3178 ELISA Kit; PDIA15 ELISA Kit; Protein disulfide isomerase family A member 15 ELISA Kit; STRF8 ELISA Kit; Thioredoxin domain containing 5 (endoplasmic reticulum) ELISA Kit; Thioredoxin domain containing 5 ELISA Kit; Thioredoxin domain containing protein 5 ELISA Kit; Thioredoxin domain-containing protein 5 ELISA Kit; Thioredoxin like protein p46 ELISA Kit; Thioredoxin related protein ELISA Kit; Thioredoxin-like protein p46 ELISA Kit; TLP46 ELISA Kit; TXND5_HUMAN ELISA Kit; TXNDC 5 ELISA Kit; Txndc5 ELISA Kit |
| Abbreviation |
TXNDC5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TXNDC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
90 |
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| Range % |
87-94 |
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| 1:10 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:20 |
Average % |
88 |
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| Range % |
80-96 |
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| 1:40 |
Average % |
92 |
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| Range % |
86-99 |
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| Recovery |
| The recovery of human TXNDC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
89-104 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.286 |
2.186 |
2.236 |
2.083 |
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| 500 |
1.752 |
1.652 |
1.702 |
1.549 |
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| 250 |
1.193 |
1.153 |
1.173 |
1.020 |
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| 125 |
0.704 |
0.694 |
0.699 |
0.546 |
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| 62.5 |
0.441 |
0.431 |
0.436 |
0.283 |
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| 31.2 |
0.311 |
0.301 |
0.306 |
0.153 |
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| 15.6 |
0.236 |
0.226 |
0.231 |
0.078 |
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| 0 |
0.153 |
0.152 |
0.153 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
