Human thrombin-antithrombin complex,TAT ELISA Kit
Product Details
| Description |
The Human thrombin-antithrombin complex (TAT) ELISA kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of TAT in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for cardiovascular research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which TAT in the samples or standards are sandwiched between pre-coated TAT antibody and Biotin-conjugated antibody specific for TAT. HRP-avidin is then added to the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of TAT bound in the initial step. The color development is st... Read more |
| Target Name |
thrombin-antithrombin complex,TAT |
| Alternative Names |
N/A |
| Abbreviation |
TAT |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
94 |
|
| Range % |
90-99 |
|
| 1:2 |
Average % |
99 |
|
| Range % |
94-103 |
|
| 1:4 |
Average % |
88 |
|
| Range % |
82-93 |
|
| 1:8 |
Average % |
95 |
|
| Range % |
91-100 |
|
|
| Recovery |
| The recovery of human TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-96 |
|
| EDTA plasma (n=4) |
98 |
92-103 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 4000 |
2.773 |
2.753 |
2.763 |
2.576 |
|
| 2000 |
2.134 |
2.178 |
2.156 |
1.969 |
|
| 1000 |
1.342 |
1.368 |
1.355 |
1.168 |
|
| 500 |
0.795 |
0.756 |
0.776 |
0.589 |
|
| 250 |
0.524 |
0.502 |
0.513 |
0.326 |
|
| 125 |
0.378 |
0.398 |
0.388 |
0.201 |
|
| 62.5 |
0.317 |
0.327 |
0.322 |
0.135 |
|
| 0 |
0.186 |
0.188 |
0.187 |
|
|
|
|
| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TAT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TAT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
