Human Thromboxane B2,TX-B2 ELISA Kit
Product Details
| Target Name |
Thromboxane B2,TX-B2 |
| Abbreviation |
TX-B2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
9.38 pg/mL-600 pg/mL |
| Sensitivity |
2.34 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TX-B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
80-105 |
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| 1:2 |
Average % |
95 |
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| Range % |
85-108 |
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| 1:4 |
Average % |
106 |
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| Range % |
90-112 |
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| 1:8 |
Average % |
95 |
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| Range % |
80-110 |
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| Recovery |
| The recovery of human TX-B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
80-110 |
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| EDTA plasma (n=4) |
98 |
90-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 600 |
3.147 |
3.121 |
3.134 |
2.997 |
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| 300 |
2.534 |
2.521 |
2.528 |
2.390 |
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| 150 |
1.750 |
1.744 |
1.747 |
1.610 |
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| 75 |
1.052 |
1.046 |
1.049 |
0.912 |
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| 37.5 |
0.659 |
0.644 |
0.652 |
0.514 |
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| 18.75 |
0.443 |
0.428 |
0.436 |
0.298 |
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| 9.37 |
0.263 |
0.284 |
0.274 |
0.136 |
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| 0 |
0.134 |
0.141 |
0.138 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
