Human thymic stromal lymphopoietin,TSLP ELISA Kit
Product Details
| Description |
CUSABIO's Human thymic stromal lymphopoietin (TSLP) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of TSLP concentrations in serum, cell culture supernates, and tissue homogenates. This assay exclusively recognizes human TSLP. The quantitative sandwich ELISA technique of this kit is based on TSLP antibody-TSLP antigen interactions and an HRP colorimetric detection system to detect the levels of TSLP in samples. The intensity of the color is positively proportional to the amount of bound TSLP in the initial step. This human TSLP ELISA kit has been quality validated for criteria, including sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. Click the product instructions for more details. TSLP is a pleiotropic cytokine originally isolated from a murine thymic stromal cell line. It is mainly produced by activated lung and intestinal epithelial cells, keratinocytes, and fibroblasts. TSLP acts by binding to a h... Read more |
| Target Name |
thymic stromal lymphopoietin |
| Alternative Names |
Thymic stromal lymphopoietin ELISA Kit; Thymic stromal lymphopoietin protein TSLP ELISA Kit; Tslp ELISA Kit; TSLP protein ELISA Kit; TSLP_HUMAN ELISA Kit |
| Abbreviation |
TSLP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, cell culture supernates, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TSLP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-101 |
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| 1:2 |
Average % |
98 |
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| Range % |
93-102 |
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| 1:4 |
Average % |
99 |
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| Range % |
94-103 |
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| 1:8 |
Average % |
92 |
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| Range % |
85-100 |
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| Recovery |
| The recovery of human TSLP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
95-101 |
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| EDTA plasma (n=4) |
90 |
87-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.799 |
2.786 |
2.793 |
2.615 |
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| 20 |
2.365 |
2.399 |
2.382 |
2.204 |
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| 10 |
1.793 |
1.782 |
1.788 |
1.610 |
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| 5 |
1.231 |
1.256 |
1.244 |
1.066 |
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| 2.5 |
0.758 |
0.768 |
0.763 |
0.585 |
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| 1.25 |
0.546 |
0.533 |
0.540 |
0.362 |
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| 0.625 |
0.366 |
0.378 |
0.372 |
0.194 |
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| 0 |
0.179 |
0.176 |
0.178 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human TSLP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TSLP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
