| Description |
The Human tight junction protein 1 (ZO-1) ELISA kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of ZO-1 in the serum, plasma, cell culture supernates, or cell lysates. It is not intended for diagnostic use. This assay kit was designed and optimized for Tags & Cell Markers research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which ZO-1 in the samples or standards are sandwiched between pre-coated ZO-1 antibody and Biotin-conjugated antibody specific for ZO-1. HRP-avidin is then added to the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of ZO-1 bound in the initial step... Read more |
| Target Name |
tight junction protein 1 (zona occludens 1) |
| Alternative Names |
Tight junction protein 1 ELISA Kit; Tight junction protein ZO-1 ELISA Kit; Tight junction protein ZO1 ELISA Kit; TJP1 ELISA Kit; zo-1 ELISA Kit; Zo1 ELISA Kit; ZO1_HUMAN ELISA Kit; Zona occludens 1 ELISA Kit; Zona occludens 1 protein ELISA Kit; Zona occludens protein 1 ELISA Kit; Zonula occludens 1 protein ELISA Kit; Zonula occludens protein 1 ELISA Kit |
| Abbreviation |
TJP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, cell lysates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Tags & Cell Markers |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
99 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
100 |
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| Range % |
92-108 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-97 |
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| EDTA plasma (n=4) |
97 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.484 |
2.467 |
2.476 |
2.329 |
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| 500 |
1.707 |
1.678 |
1.693 |
1.546 |
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| 250 |
1.076 |
1.055 |
1.066 |
0.919 |
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| 125 |
0.679 |
0.669 |
0.674 |
0.527 |
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| 62.5 |
0.441 |
0.436 |
0.439 |
0.292 |
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| 31.2 |
0.297 |
0.287 |
0.292 |
0.145 |
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| 15.6 |
0.222 |
0.209 |
0.216 |
0.069 |
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| 0 |
0.149 |
0.145 |
0.147 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ZO-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ZO-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
