Human Tissue factor pathway inhibitor,TFPI ELISA Kit
Product Details
| Target Name |
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) |
| Alternative Names |
Anti convertin ELISA Kit; EPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; LACI ELISA Kit; Lipoprotein associated coagulation inhibitor ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; TFI ELISA Kit; TFPI 1 ELISA Kit; TFPI ELISA Kit; TFPI1 ELISA Kit; TFPI1_HUMAN ELISA Kit; Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) ELISA Kit; Tissue factor pathway inhibitor ELISA Kit |
| Abbreviation |
TFPI |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.782 ng/mL-50 ng/mL |
| Sensitivity |
0.293 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:2 |
Average % |
101 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:8 |
Average % |
96 |
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| Range % |
91-102 |
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| Recovery |
| The recovery of human TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
86 |
81-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.519 |
2.623 |
2.571 |
2.440 |
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| 25 |
2.021 |
2.154 |
2.088 |
1.957 |
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| 12.5 |
1.434 |
1.456 |
1.445 |
1.314 |
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| 6.25 |
0.813 |
0.838 |
0.826 |
0.695 |
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| 3.125 |
0.436 |
0.445 |
0.441 |
0.310 |
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| 1.563 |
0.304 |
0.310 |
0.307 |
0.176 |
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| 0.782 |
0.221 |
0.216 |
0.219 |
0.088 |
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| 0 |
0.130 |
0.131 |
0.131 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
