Human Titin(TTN) ELISA kit
Product Details
| Target Name |
titin |
| Alternative Names |
MPRM ELISA Kit; Cardiomyopathy dilated 1G (autosomal dominant) ELISA Kit; CMD1G ELISA Kit; CMH 9 ELISA Kit; CMH9 ELISA Kit; CMPD 4 ELISA Kit; CMPD4 ELISA Kit; Connectin ELISA Kit; DKFZp451N061 ELISA Kit; EOMFC ELISA Kit; FLJ26020 ELISA Kit; FLJ26409 ELISA Kit; FLJ32040 ELISA Kit; FLJ34413 ELISA Kit; FLJ39564 ELISA Kit; FLJ43066 ELISA Kit; HMERF ELISA Kit; LGMD2J ELISA Kit; MU RMS 40.14 ELISA Kit; MYLK5 ELISA Kit; Rhabdomyosarcoma antigen ELISA Kit; Rhabdomyosarcoma antigen MU RMS 40.14 ELISA Kit; Rhabdomyosarcoma antigen MU-RMS-40.14 ELISA Kit; Titin ELISA Kit; TITIN_HUMAN ELISA Kit; TMD ELISA Kit; TTN ELISA Kit |
| Abbreviation |
TTN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
23.5 pg/mL-1500 pg/mL |
| Sensitivity |
5.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
94-104 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:8 |
Average % |
92 |
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| Range % |
84-98 |
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| Recovery |
| The recovery of human TTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
92-100 |
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| EDTA plasma (n=4) |
98 |
90-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1500 |
2.195 |
2.095 |
2.145 |
2.014 |
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| 750 |
1.594 |
1.494 |
1.544 |
1.413 |
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| 375 |
0.932 |
0.892 |
0.912 |
0.781 |
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| 187.5 |
0.506 |
0.486 |
0.496 |
0.365 |
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| 94 |
0.352 |
0.342 |
0.347 |
0.216 |
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| 47 |
0.229 |
0.219 |
0.224 |
0.093 |
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| 23.5 |
0.168 |
0.162 |
0.165 |
0.034 |
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| 0 |
0.131 |
0.130 |
0.131 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
