Human toll-like receptor 4,TLR4 ELISA Kit
Product Details
| Description |
The Human toll-like receptor 4 (TLR4) ELISA kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of TLR4 in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for immunology research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. The assay principle applied in this kit is the quantitative Sandwich enzyme immunoassay. The microtiter plate has been pre-coated with an anti-human TLR4 antibody. Standards or samples are added to the appropriate microtiter plate wells with the Biotin-conjugated TLR4 antibody. Next, HRP-Avidin is added to each microplate well. After TMB substrate solution is added, wells containing TLR4, Biotin-conjugated TLR4 antibody, and HRP-conjugated Avidin will exhibit a c... Read more |
| Target Name |
toll-like receptor 4 |
| Alternative Names |
ARMD10 ELISA Kit; CD284 ELISA Kit; CD284 antigen ELISA Kit; ESOP 1 ELISA Kit; ESOP1 ELISA Kit; Homolog of Drosophila toll ELISA Kit; hToll ELISA Kit; LY 96 ELISA Kit; LY96 ELISA Kit; Lymphocyte antigen 96 ELISA Kit; md 2 ELISA Kit; MD 2 protein ELISA Kit; MD2 protein ELISA Kit; Myeloid differentiation protein 2 ELISA Kit; Protein MD 2 ELISA Kit; Protein MD2 ELISA Kit; TLR 4 ELISA Kit; TLR4 ELISA Kit; TLR4_HUMAN ELISA Kit; TOLL ELISA Kit; Toll like receptor 4 ELISA Kit; Toll-like receptor 4 ELISA Kit |
| Abbreviation |
TLR4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
85 |
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| Range % |
80-93 |
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| 1:10 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:20 |
Average % |
97 |
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| Range % |
93-104 |
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| 1:40 |
Average % |
95 |
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| Range % |
91-99 |
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| Recovery |
| The recovery of human TLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
89-103 |
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| EDTA plasma (n=4) |
97 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.463 |
2.547 |
2.505 |
2.372 |
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| 5 |
2.121 |
2.172 |
2.147 |
2.014 |
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| 2.5 |
1.540 |
1.516 |
1.528 |
1.395 |
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| 1.25 |
0.725 |
0.747 |
0.736 |
0.603 |
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| 0.625 |
0.418 |
0.436 |
0.427 |
0.294 |
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| 0.312 |
0.236 |
0.241 |
0.239 |
0.106 |
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| 0.156 |
0.199 |
0.206 |
0.203 |
0.070 |
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| 0 |
0.132 |
0.134 |
0.133 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TLR4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TLR4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
