Human transglutaminase 2C polypeptide,TGM2 ELISA Kit
Product Details
| Target Name |
transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) |
| Alternative Names |
ALPHA SUBUNIT ELISA Kit; C polypeptide ELISA Kit; EC 2.3.2.13 ELISA Kit; epididymis secretory protein Li 45 ELISA Kit; G alpha h ELISA Kit; G[a]h ELISA Kit; Gh CLASS G ALPHA h ELISA Kit; GNAH ELISA Kit; GNAH G PROTEIN ELISA Kit; H POLYPEPTIDE ELISA Kit; HEL-S-45 ELISA Kit; Protein glutamine gamma glutamyltransferase 2 ELISA Kit; Protein-glutamine gamma-glutamyltransferase 2 ELISA Kit; TG 2 ELISA Kit; TG(C) ELISA Kit; TG2 ELISA Kit; TGase C ELISA Kit; TGase H ELISA Kit; TGase-2 ELISA Kit; TgaseII ELISA Kit; TGC ELISA Kit; TGC GUANINE NUCLEOTIDE BINDING PROTEIN ELISA Kit; TGM2 ELISA Kit; TGM2_HUMAN ELISA Kit; Tissue transglutaminase ELISA Kit; Transglutaminase 2 ELISA Kit; Transglutaminase 2 C polypeptide ELISA Kit; Transglutaminase C ELISA Kit; Transglutaminase H ELISA Kit; Transglutaminase-2 ELISA Kit; tTG ELISA Kit; tTGas ELISA Kit |
| Abbreviation |
TGM2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
18.75 pg/mL-1200 pg/mL |
| Sensitivity |
4.68 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TGM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:5 |
Average % |
86 |
|
| Range % |
81-91 |
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| 1:10 |
Average % |
99 |
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| Range % |
93-102 |
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| 1:20 |
Average % |
99 |
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| Range % |
93-105 |
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| 1:40 |
Average % |
92 |
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| Range % |
86-95 |
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| Recovery |
| The recovery of human TGM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
89-97 |
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| EDTA plasma (n=4) |
97 |
93-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1200 |
2.736 |
2.654 |
2.695 |
2.563 |
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| 600 |
2.413 |
2.321 |
2.367 |
2.235 |
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| 300 |
1.908 |
1.854 |
1.881 |
1.749 |
|
| 150 |
0.967 |
0.988 |
0.978 |
0.846 |
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| 75 |
0.698 |
0.702 |
0.700 |
0.568 |
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| 37.5 |
0.388 |
0.413 |
0.401 |
0.269 |
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| 18.75 |
0.277 |
0.264 |
0.271 |
0.139 |
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| 0 |
0.134 |
0.129 |
0.132 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
