Human Tropomyosin alpha-4 chain(TPM4) ELISA kit
Product Details
| Target Name |
tropomyosin 4 |
| Alternative Names |
epididymis secretory protein Li 108 ELISA Kit; HEL-S-108 ELISA Kit; TM30p1 ELISA Kit; TMP 4 ELISA Kit; TPM4 ELISA Kit; TPM4_HUMAN ELISA Kit; Tropomyosin 4 ELISA Kit; Tropomyosin alpha 4 chain ELISA Kit; Tropomyosin alpha-4 chain ELISA Kit; Tropomyosin-4 ELISA Kit; wu:fb06e07 ELISA Kit; zgc:103436 ELISA Kit; zgc:63909 ELISA Kit |
| Abbreviation |
TPM4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
23.5 pg/mL-1500 pg/mL |
| Sensitivity |
5.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TPM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:2 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:4 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
95-104 |
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| Recovery |
| The recovery of human TPM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
87 |
82-90 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1500 |
2.557 |
2.597 |
2.577 |
2.398 |
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| 750 |
1.916 |
1.936 |
1.926 |
1.747 |
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| 375 |
1.278 |
1.298 |
1.288 |
1.109 |
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| 187.5 |
0.798 |
0.742 |
0.770 |
0.591 |
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| 94 |
0.481 |
0.499 |
0.490 |
0.311 |
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| 47 |
0.367 |
0.355 |
0.361 |
0.182 |
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| 23.5 |
0.264 |
0.268 |
0.266 |
0.087 |
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| 0 |
0.178 |
0.179 |
0.179 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
