| Description |
The product CSB-EL023959HU is a ready-to-use microwell, strip plate ELISA Kit for quantitative detection of tumor necrosis factor-inducible gene 6 protein (TNFAIP6) levels in human serum, plasma, or cell culture supernates. This kit The kit has undergone multiple validations, including sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. Refer to the product instructions for more details. The detection mechanism of this assay is based on the sandwich ELISA technique. TNFAIP6 in the samples or standards is sandwiched between pre-coated TNFAIP6 antibody and Biotin-conjugated TNFAIP6 antibody, forming an Ab-Ag-Ab complex. The pre-coated TNFAIP6 antibody/TNFAIP6/Biotin-conjugated TNFAIP6 antibody complex is labeled with HRP and then triggered a chromogenic reaction after the addition of the TMB substrate solution. The color develops in proportion to the amount of TNFAIP6 in the samples. The color development is terminated upon adding the stop solution, and ... Read more |
| Target Name |
tumor necrosis factor, alpha-induced protein 6 |
| Alternative Names |
Hyaluronate binding protein ELISA Kit; Hyaluronate-binding protein ELISA Kit; TNF alpha induced protein 6 ELISA Kit; TNF alpha-induced protein 6 ELISA Kit; TNF stimulated gene 6 protein ELISA Kit; TNF-stimulated gene 6 protein ELISA Kit; TNFAIP 6 ELISA Kit; Tnfaip6 ELISA Kit; TSG 6 ELISA Kit; TSG-6 ELISA Kit; TSG6_HUMAN ELISA Kit; Tumor necrosis factor alpha induced protein 6 ELISA Kit; Tumor necrosis factor alpha inducible protein 6 ELISA Kit; Tumor necrosis factor alpha-induced protein 6 ELISA Kit; Tumor necrosis factor inducible gene 6 protein ELISA Kit; Tumor necrosis factor stimulated gene 6 protein ELISA Kit; Tumor necrosis factor-inducible gene 6 protein ELISA Kit |
| Abbreviation |
TNFAIP6 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNFAIP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
96 |
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| Range % |
86-98 |
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| 1:10 |
Average % |
87 |
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| Range % |
84-93 |
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| 1:20 |
Average % |
98 |
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| Range % |
92-103 |
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| 1:40 |
Average % |
89 |
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| Range % |
84-99 |
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| Recovery |
| The recovery of human TNFAIP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
88-98 |
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| EDTA plasma (n=4) |
94 |
92-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.792 |
2.787 |
2.790 |
2.679 |
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| 5 |
2.136 |
2.122 |
2.129 |
2.018 |
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| 2.5 |
1.378 |
1.334 |
1.356 |
1.245 |
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| 1.25 |
0.779 |
0.753 |
0.766 |
0.655 |
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| 0.625 |
0.365 |
0.355 |
0.360 |
0.249 |
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| 0.312 |
0.274 |
0.270 |
0.272 |
0.161 |
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| 0.156 |
0.231 |
0.218 |
0.225 |
0.114 |
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| 0 |
0.114 |
0.108 |
0.111 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TNFAIP6 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TNFAIP6 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
