Human tumor necrosis factor receptor-associated factor 6,TRAF6 ELISA kit
Product Details
| Target Name |
TNF receptor-associated factor 6 |
| Alternative Names |
E3 ubiquitin-protein ligase TRAF6 ELISA Kit; Interleukin 1 signal transducer ELISA Kit; Interleukin-1 signal transducer ELISA Kit; MGC 3310 ELISA Kit; MGC:3310 ELISA Kit; MGC3310 ELISA Kit; OTTHUMP00000232772 ELISA Kit; OTTHUMP00000232773 ELISA Kit; RING finger protein 85 ELISA Kit; RNF 85 ELISA Kit; RNF85 ELISA Kit; TNF receptor associated factor 6 ELISA Kit; TNF receptor-associated factor 6 ELISA Kit; TNF receptor-associated factor 6; E3 ubiquitin protein ligase ELISA Kit; TRAF 6 ELISA Kit; Traf6 ELISA Kit; TRAF6_HUMAN ELISA Kit |
| Abbreviation |
TRAF6 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
39 pg/mL-2500 pg/mL |
| Sensitivity |
9.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRAF6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
|
| Range % |
87-99 |
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| 1:2 |
Average % |
103 |
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| Range % |
99-104 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-98 |
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| 1:8 |
Average % |
91 |
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| Range % |
89-97 |
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| Recovery |
| The recovery of human TRAF6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
92-99 |
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| EDTA plasma (n=4) |
90 |
86-93 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 2500 |
2.444 |
2.246 |
2.345 |
2.239 |
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| 1250 |
1.937 |
1.780 |
1.859 |
1.753 |
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| 625 |
1.432 |
1.544 |
1.488 |
1.382 |
|
| 312.5 |
1.078 |
1.159 |
1.119 |
1.013 |
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| 156 |
0.726 |
0.760 |
0.743 |
0.637 |
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| 78 |
0.376 |
0.358 |
0.367 |
0.261 |
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| 39 |
0.261 |
0.244 |
0.253 |
0.147 |
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| 0 |
0.103 |
0.109 |
0.106 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
