Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA) ELISA kit
Product Details
| Target Name |
signal-regulatory protein alpha |
| Alternative Names |
Signal regulatory protein alpha type 1 ELISA Kit; Bit ELISA Kit; Brain Ig like molecule with tyrosine based activation motifs ELISA Kit; Brain Ig-like molecule with tyrosine-based activation motifs ELISA Kit; Brain immunoglobulin like molecule with tyrosine based activation motifs ELISA Kit; CD172 antigen like family member A ELISA Kit; CD172 antigen-like family member A ELISA Kit; CD172a ELISA Kit; CD172a antigen ELISA Kit; Inhibitory receptor SHPS-1 ELISA Kit; Macrophage fusion receptor ELISA Kit; MFR ELISA Kit; MYD 1 ELISA Kit; Myd 1 antigen ELISA Kit; MyD-1 antigen ELISA Kit; p84 ELISA Kit; Protein tyrosine phosphatase non receptor type substrate 1 ELISA Kit; PTPNS1 ELISA Kit; SHP substrate 1 ELISA Kit; SHPS-1 ELISA Kit; SHPS1 ELISA Kit; SHPS1_HUMAN ELISA Kit; Signal regulatory protein alpha 2 ELISA Kit; Signal regulatory protein alpha 3 ELISA Kit; Signal regulatory protein alpha ELISA Kit; Signal regulatory protein alpha type 2 ELISA Kit; Signal-regulatory protein alpha-1 ELISA Kit; Signal-regulatory protein alpha-2 ELISA Kit; Signal-regulatory protein alpha-3 ELISA Kit; SIRP ELISA Kit; Sirp-alpha-1 ELISA Kit; Sirp-alpha-2 ELISA Kit; Sirp-alpha-3 ELISA Kit; SIRPA ELISA Kit; SIRPalpha ELISA Kit; SIRPalpha1 ELISA Kit; SIRPalpha2 ELISA Kit; SIRPalpha3 ELISA Kit; Tyrosine phosphatase SHP substrate 1 ELISA Kit; Tyrosine protein phosphatase non receptor type substrate 1 ELISA Kit; Tyrosine-protein phosphatase non-receptor type substrate 1 ELISA Kit |
| Abbreviation |
SIRPA |
| Uniprot No. |
P78324 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SIRPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
90 |
|
| Range % |
85-96 |
|
| 1:2 |
Average % |
96 |
|
| Range % |
91-105 |
|
| 1:4 |
Average % |
98 |
|
| Range % |
95-106 |
|
| 1:8 |
Average % |
86 |
|
| Range % |
80-92 |
|
|
| Recovery |
| The recovery of human SIRPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
86-96 |
|
| EDTA plasma (n=4) |
92 |
88-97 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.228 |
2.128 |
2.178 |
2.064 |
|
| 5 |
1.527 |
1.458 |
1.493 |
1.379 |
|
| 2.5 |
0.868 |
0.838 |
0.853 |
0.739 |
|
| 1.25 |
0.427 |
0.417 |
0.422 |
0.308 |
|
| 0.625 |
0.298 |
0.288 |
0.293 |
0.179 |
|
| 0.312 |
0.220 |
0.217 |
0.219 |
0.105 |
|
| 0.156 |
0.174 |
0.170 |
0.172 |
0.058 |
|
| 0 |
0.115 |
0.112 |
0.114 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
