Human Tyrosyl-DNA Phosphodiesterase 1(TDP1)ELISA Kit
Product Details
| Target Name |
tyrosyl-DNA phosphodiesterase 1 |
| Alternative Names |
AI838772 ELISA Kit; AW493413 ELISA Kit; FLJ11090 ELISA Kit; MGC104252 ELISA Kit; MGC112732 ELISA Kit; RP24-311F12.2 ELISA Kit; SCAN1 ELISA Kit; TDP1 ELISA Kit; TYDP ELISA Kit; TYDP1_HUMAN ELISA Kit; Tyr-DNA phosphodiesterase 1 ELISA Kit; Tyrosyl-DNA phosphodiesterase 1 ELISA Kit |
| Abbreviation |
TDP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Epigenetics and Nuclear Signaling |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TDP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-100 |
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| 1:2 |
Average % |
105 |
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| Range % |
100-112 |
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| 1:4 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-94 |
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| Recovery |
| The recovery of human TDP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
91 |
86-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.418 |
2.520 |
2.469 |
2.314 |
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| 2000 |
1.960 |
1.976 |
1.968 |
1.813 |
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| 1000 |
1.443 |
1.504 |
1.474 |
1.319 |
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| 500 |
0.884 |
0.939 |
0.912 |
0.757 |
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| 250 |
0.593 |
0.634 |
0.614 |
0.459 |
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| 125 |
0.426 |
0.449 |
0.438 |
0.283 |
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| 62.5 |
0.312 |
0.309 |
0.311 |
0.156 |
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| 0 |
0.158 |
0.152 |
0.155 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
