Human ubiquitin-like modifier (ISG15) ELISA kit
Product Details
| Description |
The product CSB-E12075h is a ready-to-use microwell, strip plate ELISA Kit for quantitatively analyzing the amount of the ubiquitin-like modifier (ISG15) in human serum, tissue homogenates, or cell culture supernates. ISG15 is a ubiquitin‐like protein strongly induced by type I interferons (IFNs). ISG15 is covalently conjugated to the bulk of newly synthesized polypeptides at the ribosome through ISGylation. Viral and bacterial infections, lipopolysaccharide (LPS), retinoic acid, or certain genotoxic stressors, also induce the expression of ISG15. This shows that ISG15 is involved in the host antiviral response. ISG15 functions as an antiviral molecule through direct inhibition of viral replication and regulation of host immunity. This assay is based on the Sandwich-ELISA mechanism (the ISG15 in the sample is sandwiched between the pre-coated ISG15 antibody and Biotin-labeled ISG15 antibody) and enzyme-substrate chromogenic reaction. The color intensity is positively related to the amo... Read more |
| Target Name |
ISG15 ubiquitin-like modifier |
| Alternative Names |
G1P2 ELISA Kit; hUCRP ELISA Kit; IFI 15 ELISA Kit; IFI15 ELISA Kit; Interferon alpha inducible protein ELISA Kit; Interferon induced protein 15 ELISA Kit; Interferon induced protein IFI-15K ELISA Kit; Interferon stimulated protein 15 kDa ELISA Kit; Interferon; alpha-inducible protein (clone IFI-15K) ELISA Kit; Interferon-induced 15 kDa protein ELISA Kit; Interferon-induced 17 kDa protein ELISA Kit; Interferon-induced 17-kDa/15-kDa protein ELISA Kit; IP17 ELISA Kit; ISG 15 ELISA Kit; ISG15 ELISA Kit; ISG15 ubiquitin like modifier ELISA Kit; ISG15_HUMAN ELISA Kit; Ubiquitin cross-reactive protein ELISA Kit; Ubiquitin-like protein ISG15 ELISA Kit; UCRP ELISA Kit |
| Abbreviation |
ISG15 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, tissue homogenates, cell culture supernates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
97 |
|
| Range % |
90-105 |
|
| 1:2 |
Average % |
90 |
|
| Range % |
84-95 |
|
| 1:4 |
Average % |
99 |
|
| Range % |
95-102 |
|
| 1:8 |
Average % |
97 |
|
| Range % |
92-104 |
|
|
| Recovery |
| The recovery of human ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-104 |
|
| EDTA plasma (n=4) |
103 |
98-109 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.785 |
2.799 |
2.792 |
2.636 |
|
| 25 |
2.457 |
2.498 |
2.478 |
2.322 |
|
| 12.5 |
1.982 |
1.999 |
1.991 |
1.835 |
|
| 6.25 |
1.272 |
1.291 |
1.282 |
1.126 |
|
| 3.12 |
0.775 |
0.798 |
0.787 |
0.631 |
|
| 1.56 |
0.533 |
0.503 |
0.518 |
0.362 |
|
| 0.78 |
0.331 |
0.356 |
0.344 |
0.188 |
|
| 0 |
0.155 |
0.157 |
0.156 |
|
|
|
|
| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ISG15 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ISG15 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
