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Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT

Product Details

Target Name vascular endothelial growth factor A
Alternative Names glioma-derived endothelial cell mitogen ELISA Kit; MGC70609 ELISA Kit; MVCD1 ELISA Kit; Vascular endothelial growth factor A ELISA Kit; Vascular Endothelial Growth Factor ELISA Kit; Vascular permeability factor ELISA Kit; VEGF ELISA Kit; VEGF-A ELISA Kit; VEGF165 ELISA Kit; Vegfa ELISA Kit; VEGFA_HUMAN ELISA Kit; VPF ELISA Kit
Abbreviation VEGFA
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 25.297 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 94-106  
1:2 Average % 93  
Range % 86-97  
1:4 Average % 87  
Range % 82-93  
1:8 Average % 96  
Range % 91-105  
Recovery
The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 92 90-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.651 2.751 2.701 2.547  
1000 1.729 1.829 1.779 1.625  
500 1.067 1.087 1.077 0.923  
250 0.579 0.589 0.584 0.430  
125 0.372 0.382 0.377 0.223  
62.5 0.255 0.265 0.260 0.106  
31.25 0.207 0.209 0.208 0.054  
0 0.153 0.154 0.154    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days