Human Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit
Product Details
| Target Name |
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 |
| Alternative Names |
ENSG00000165953 ELISA Kit; OL 64 ELISA Kit; OL-64 ELISA Kit; OL64 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 ELISA Kit; Serpin A12 ELISA Kit; Serpin A12 precursor ELISA Kit; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 ELISA Kit; Serpina12 ELISA Kit; SPA12_HUMAN ELISA Kit; Vaspin ELISA Kit; Visceral adipose specific serpin ELISA Kit; Visceral adipose tissue derived serine protease inhibitor ELISA Kit; Visceral adipose tissue-derived serine protease inhibitor ELISA Kit; Visceral adipose-specific serpin ELISA Kit |
| Abbreviation |
SERPINA12 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
100 |
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| Range % |
88-115 |
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| 1:10 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:20 |
Average % |
99 |
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| Range % |
92-112 |
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| 1:40 |
Average % |
93 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of human vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-98 |
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| EDTA plasma (n=4) |
96 |
88-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.717 |
2.758 |
2.738 |
2.559 |
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| 100 |
2.305 |
2.356 |
2.331 |
2.152 |
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| 50 |
1.736 |
1.756 |
1.746 |
1.567 |
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| 25 |
1.043 |
1.084 |
1.064 |
0.885 |
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| 12.5 |
0.609 |
0.618 |
0.614 |
0.435 |
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| 6.25 |
0.452 |
0.457 |
0.455 |
0.276 |
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| 3.12 |
0.326 |
0.345 |
0.336 |
0.157 |
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| 0 |
0.182 |
0.175 |
0.179 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
