| Description |
CUSABIO's Human visfatin ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of visfatin levels in serum, plasma, urine, and tissue homogenates. This assay exclusively recognizes human visfatin. The quantitative sandwich ELISA technique of this kit is based on visfatin antibody-visfatin antigen interactions and an HRP colorimetric detection system to detect the levels of visfatin in samples. The intensity of the color is positively proportional to the amount of bound visfatin in the initial step.
This human visfatin ELISA kit has been quality validated for criteria, including sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. Click the product instructions for more details.
Visfatin, also known as nicotinamide phosphoribosyltransferase (NAMPT), is a multifaceted adipokine mainly secreted by the visceral adipose tissue. As a NAD biosynthetic enzyme, NAMPT catalyzes the transformation of nicotinamide into nicotinamide mononucleotide (NMN), which is then converted into NAD+ by nicotinamide/nicotinic acid mononucleotide adenylyltransferase (Nmnat). In addition to its NAD+ biosynthetic activity, NAMPT also acts as a pro-inflammatory cytokine and plays a crucial role in chronic diseases. It also exerts insulin-mimetic effects. Previous studies have shown that NAMPT is effective in activating the insulin signaling cascade, increasing glucose uptake, and inhibiting glucose release. NAMPT is related to glucose metabolism and the pathogenesis of insulin resistance and type II diabetes. NAMPT also causes in vitro endothelial dysfunction and vascular inflammation.
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| Target Name |
nicotinamide phosphoribosyltransferase |
| Alternative Names |
1110035O14Rik ELISA Kit; AI314458 ELISA Kit; AI480535 ELISA Kit; DKFZP666B131 ELISA Kit; EC 2.4.2.12 ELISA Kit; MGC117256 ELISA Kit; NAmPRTase ELISA Kit; Nampt ELISA Kit; NAMPT_HUMAN ELISA Kit; Nicotinamide phosphoribosyltransferase ELISA Kit; PBEF 1 ELISA Kit; PBEF ELISA Kit; PBEF1 ELISA Kit; Pre B cell colony enhancing factor 1 ELISA Kit; Pre B cell colony enhancing factor ELISA Kit; Pre B cell enhancing factor ELISA Kit; Pre-B cell-enhancing factor ELISA Kit; Pre-B-cell colony-enhancing factor 1 ELISA Kit; VF ELISA Kit; Visfatin ELISA Kit |
| Abbreviation |
NAMPT |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:4 |
Average % |
98 |
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| Range % |
93-102 |
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| 1:8 |
Average % |
102 |
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| Range % |
98-106 |
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| Recovery |
| The recovery of human visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
95-104 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
1.977 |
2.050 |
2.014 |
1.921 |
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| 20 |
1.526 |
1.584 |
1.555 |
1.462 |
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| 10 |
1.087 |
1.101 |
1.094 |
1.001 |
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| 5 |
0.658 |
0.703 |
0.681 |
0.588 |
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| 2.5 |
0.451 |
0.421 |
0.436 |
0.343 |
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| 1.25 |
0.293 |
0.301 |
0.297 |
0.204 |
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| 0.625 |
0.165 |
0.159 |
0.162 |
0.069 |
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| 0 |
0.096 |
0.089 |
0.093 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NAMPT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled NAMPT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
