| Description |
The Human Vitamin D Receptor (VDR) ELISA Kit can quantitatively detect the content of VDR in human serum, plasma, tissue homogenates, or cell lysates. VDR is a member of the nuclear receptor/steroid hormone receptor superfamily. It is the single known regulatory mediator of hormonal 1,25-dihydroxy vitamin D3 [1,25(OH)2D3] in higher vertebrates. It acts in the nucleus of vitamin D target cells to regulate the expression of genes whose products control diverse, cell type-specific biological functions that include mineral homeostasis. VDR is also involved in the regulation of calcium and phosphate homeostasis in tissues, including the intestine, bone, kidney, and parathyroid glands. Importantly, It participates in bone development.
This assay employs the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction to achieve the measurement. The solution develops in proportion to the amount of VDR in the sample. And the color intensity can be measured at 450 nm through a microplate reader. The kit has undergone rigorous quality validation in many aspects, including sensitivity, specificity, precision, recovery, linearity, and consistency between batches.
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| Target Name |
vitamin D (1,25- dihydroxyvitamin D3) receptor |
| Alternative Names |
1 25 dihydroxyvitamin D3 receptor ELISA Kit; 1 ELISA Kit; 1,25 dihydroxyvitamin D3 receptor ELISA Kit; 1,25-@dihydroxyvitamin D3 receptor ELISA Kit; 25-dihydroxyvitamin D3 receptor ELISA Kit; Member 1 ELISA Kit; NR1I1 ELISA Kit; Nuclear receptor subfamily 1 group I member 1 ELISA Kit; PPP1R163 ELISA Kit; Protein phosphatase 1, regulatory subunit 163 ELISA Kit; VDR ELISA Kit; VDR_HUMAN ELISA Kit; Vitamin D (1,25- dihydroxyvitamin D3) receptor ELISA Kit; Vitamin D hormone receptor ELISA Kit; Vitamin D nuclear receptor variant 1 ELISA Kit; Vitamin D receptor ELISA Kit; Vitamin D3 receptor ELISA Kit |
| Abbreviation |
VDR |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
87-96 |
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| 1:2 |
Average % |
103 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-98 |
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| Recovery |
| The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
82-94 |
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| EDTA plasma (n=4) |
99 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.178 |
2.098 |
2.138 |
1.977 |
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| 200 |
1.562 |
1.644 |
1.603 |
1.442 |
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| 100 |
1.138 |
1.118 |
1.128 |
0.967 |
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| 50 |
0.811 |
0.803 |
0.807 |
0.646 |
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| 25 |
0.452 |
0.460 |
0.456 |
0.295 |
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| 12.5 |
0.325 |
0.315 |
0.320 |
0.159 |
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| 6.25 |
0.227 |
0.231 |
0.229 |
0.068 |
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| 0 |
0.162 |
0.160 |
0.161 |
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| Materials provided |
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human VDR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labled VDR antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
