| Description |
The Mouse Acetylcholinesterase (AChE) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of mouse AChE in many biological fluids, including serum, plasma, cell culture supernates, and tissue homogenates. The kit has high sensitivity, excellent specificity, good linearity, precision low than 10%, high recovery, good lot-to-lot consistency. Get access to more details from the product instructions. This assay employs the Sandwich-ELISA technique, in which AChE in the samples or standards is sandwiched between pre-coated anti-mouse AChE and Biotin-labeled AChE antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of AChE in the sample. AChE is a cholinergic enzyme primarily present at postsynaptic neuromuscular junctions, particularly in muscles and nerves. It is responsible for the hydrolytic degradation of... Read more |
| Target Name |
Acetylcholinesterase(AChE) |
| Alternative Names |
Ache ELISA Kit; Acetylcholinesterase ELISA Kit; AChE ELISA Kit; EC 3.1.1.7 ELISA Kit |
| Abbreviation |
AChE |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.312 mU/mL-20 mU/mL |
| Sensitivity |
0.078 mU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
90 |
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| Range % |
84-96 |
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| 1:200 |
Average % |
96 |
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| Range % |
91-104 |
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| 1:400 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:800 |
Average % |
95 |
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| Range % |
88-102 |
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| Recovery |
| The recovery of mouse AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-101 |
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| EDTA plasma (n=4) |
98 |
92-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.313 |
2.413 |
2.363 |
2.260 |
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| 10 |
1.498 |
1.598 |
1.548 |
1.445 |
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| 5 |
0.840 |
0.830 |
0.835 |
0.732 |
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| 2.5 |
0.479 |
0.489 |
0.484 |
0.381 |
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| 1.25 |
0.306 |
0.316 |
0.311 |
0.208 |
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| 0.625 |
0.197 |
0.207 |
0.202 |
0.099 |
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| 0.312 |
0.135 |
0.146 |
0.141 |
0.038 |
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| 0 |
0.103 |
0.103 |
0.103 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse AChE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled AChE antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
