Mouse Angiotensin 1-7(Ang1-7)ELISA Kit
Product Details
| Target Name |
Angiotensin 1-7(Ang1-7) |
| Abbreviation |
Ang1-7 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-95 |
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| 1:2 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-102 |
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| 1:8 |
Average % |
95 |
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| Range % |
87-98 |
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| Recovery |
| The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
92-98 |
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| EDTA plasma (n=4) |
95 |
91-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.745 |
2.721 |
2.733 |
2.597 |
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| 200 |
2.316 |
2.245 |
2.281 |
2.145 |
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| 100 |
1.923 |
1.898 |
1.911 |
1.775 |
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| 50 |
1.467 |
1.366 |
1.417 |
1.281 |
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| 25 |
0.796 |
0.788 |
0.792 |
0.656 |
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| 12.5 |
0.458 |
0.447 |
0.453 |
0.317 |
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| 6.25 |
0.258 |
0.242 |
0.250 |
0.114 |
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| 0 |
0.138 |
0.134 |
0.136 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
