Mouse anti-nuclear Antibody (IgG) ELISA Kit
Product Details
| Description |
The Mouse anti-nuclear Antibody (IgG) ELISA kit is specifically designed and validated for the quantitative detection of anti-nuclear Antibody (IgG) in the serum and plasma. It is not intended for diagnostic use and only used for scientific research use in mice. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. The microtiter plate has been pre-coated with an anti-mouse IgG antibody. Standards or samples are added to the appropriate microtiter plate wells with the Biotin-conjugated antigen. HRP-avidin is added to each microplate well. Only those wells that contain anti-mouse IgG antibody, biotin-conjugated antigen, and HRP-Avidin will exhibit a change in color after the addition of the TMB solution. The enzyme-substrate reaction is terminated upon adding the stop solution, and the color change is measured via a micropla... Read more |
| Target Name |
anti-nuclear Antibody ( IgG) |
| Abbreviation |
ANA Ab (IgG) |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Capture ELISA |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-nuclear antibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:200 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:400 |
Average % |
85 |
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| Range % |
82-89 |
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| 1:800 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of mouse anti-nuclear antibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
92 |
87-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.751 |
2.752 |
2.752 |
2.559 |
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| 250 |
2.327 |
2.377 |
2.352 |
2.159 |
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| 125 |
1.700 |
1.675 |
1.688 |
1.495 |
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| 62.5 |
0.993 |
0.968 |
0.981 |
0.788 |
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| 31.2 |
0.637 |
0.625 |
0.631 |
0.438 |
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| 15.6 |
0.401 |
0.416 |
0.409 |
0.216 |
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| 7.8 |
0.299 |
0.289 |
0.294 |
0.101 |
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| 0 |
0.188 |
0.197 |
0.193 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse IgG antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled antigen(100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
