Mouse anti-Smith antibody ELISA Kit
Product Details
| Target Name |
anti-Smith antibody |
| Abbreviation |
Sm Ab |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-smith antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
81-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
93 |
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| Range % |
92-101 |
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| 1:8 |
Average % |
93 |
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| Range % |
82-98 |
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| Recovery |
| The recovery of mouse anti-smith antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
85-99 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.884 |
2.752 |
2.818 |
2.614 |
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| 20 |
2.345 |
2.276 |
2.311 |
2.107 |
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| 10 |
1.552 |
1.495 |
1.524 |
1.320 |
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| 5 |
0.836 |
0.801 |
0.819 |
0.615 |
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| 2.5 |
0.527 |
0.524 |
0.526 |
0.322 |
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| 1.25 |
0.321 |
0.344 |
0.333 |
0.129 |
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| 0.625 |
0.255 |
0.259 |
0.257 |
0.053 |
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| 0 |
0.201 |
0.206 |
0.204 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
