Mouse apolipoprotein A1(Apo-A1) ELISA Kit
Product Details
| Target Name |
apolipoprotein A-I |
| Alternative Names |
Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit |
| Abbreviation |
APOA1 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, urine, tissue homogenates |
| Detection Range |
31.25 ng/mL-2000 ng/mL |
| Sensitivity |
7.81 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:2000 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:4000 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:8000 |
Average % |
97 |
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| Range % |
92-101 |
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| Recovery |
| The recovery of mouse Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
93-104 |
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| EDTA plasma (n=4) |
99 |
95-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.214 |
2.256 |
2.235 |
2.062 |
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| 1000 |
1.856 |
1.890 |
1.873 |
1.700 |
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| 500 |
1.461 |
1.400 |
1.431 |
1.258 |
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| 250 |
0.964 |
0.999 |
0.982 |
0.809 |
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| 125 |
0.689 |
0.672 |
0.681 |
0.508 |
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| 62.5 |
0.481 |
0.491 |
0.486 |
0.313 |
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| 31.25 |
0.350 |
0.368 |
0.359 |
0.186 |
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| 0 |
0.172 |
0.174 |
0.173 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
