Mouse Apolipoprotein A5(apo-A5)ELISA Kit
Product Details
| Target Name |
apolipoprotein A-V |
| Alternative Names |
Apoa5 ELISA Kit; Rap3Apolipoprotein A-V ELISA Kit; Apo-AV ELISA Kit; ApoA-V ELISA Kit; Apolipoprotein A5 ELISA Kit; Regeneration-associated protein 3 ELISA Kit |
| Abbreviation |
APOA5 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates_x000D_ |
| Detection Range |
31.2 pg/mL-2000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-A5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
92 |
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| Range % |
86-95 |
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| 1:2000 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:4000 |
Average % |
93 |
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| Range % |
88-98 |
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| 1:8000 |
Average % |
89 |
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| Range % |
84-96 |
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| Recovery |
| The recovery of mouse apo-A5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
87-94 |
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| EDTA plasma (n=4) |
108 |
103-112 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.342 |
2.266 |
2.304 |
2.183 |
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| 500 |
1.966 |
1.941 |
1.954 |
1.833 |
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| 250 |
1.446 |
1.440 |
1.443 |
1.322 |
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| 125 |
0.788 |
0.770 |
0.779 |
0.658 |
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| 62.5 |
0.593 |
0.563 |
0.578 |
0.457 |
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| 31.2 |
0.357 |
0.360 |
0.359 |
0.238 |
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| 15.6 |
0.262 |
0.255 |
0.259 |
0.138 |
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| 0 |
0.123 |
0.119 |
0.121 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
