The sensitive quantitative measurement of mouse Apolipoprotein B (ApoB) in serum, plasma, and tissue homogenates is easily performed with this 96 well strip format ELISA kit. This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ApoB. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated antibody specific for ApoB. The competitive inhibition reaction is launched between with pre-coated ApoB and ApoB in samples or standards for HRP-conjugated ApoB antibody. After thorough washing, the TMB substrate solution is added to the wells, and the color develops in opposite to the amount of ApoB in the sample. The color development is stopped upon the addition of the stop solution and the intensity of the color is measured by a microplate reader at 450 nm.

This mouse ApoB ELISA Kit has high sensitivity and excellent specificity for detection of ApoB. And it also has been validated with precision low than 10%, good linearity, high recovery, and lot-to-lot consistency. Refer to the product instructions for more details.

ApoB is a structural protein that participates in lipid metabolism and constitutes lipoproteins such as very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), and low-density lipoprotein (LDL). ApoB level corresponds to the total number of VLDL, IDL, and LDL particles because each of these lipoproteins carries one ApoB molecule. And VLDL, IDL, and LDL are proved atherogenic. Consequently, the ApoB level can reflect these lipoproteins' atherogenic potential. ApoB levels are applied to evaluate the risk of cardiovascular disease.