Product Details

Description

The ELISA kit CSB-EL002005MO is applied to quantitatively detect the content of the Arginase-1 (ARG1) in samples of serum, plasma, and tissue homogenates. The detection mechanism of this mouse ARG1 ELISA kit is based on the sandwich assay. The standards or samples are added to the microtiter plate wells pre-coated with ARG1 specific antibody. Biotin-labeled ARG1 antibody and HRP-avidin are successively pipped into the wells, forming the immune complex. The solution develops color after the addition of TMB substrate. The content of ARG1 can be quantitatively determined by measuring the optical density of color with a microplate reader. This ELISA kit has been verified with high sensitivity, excellent specificity, a precision of less than 10%, good correlation and linearity, and high recovery. ARG1 is a cytosolic enzyme constitutively expressed in the liver cells. It is responsible for the hydrolysis of arginine to ornithine and urea and plays a major role in the hepatic urea cycle. The ...Read more

Target Name

arginase, liver

Alternative Names

Arg1Arginase-1 ELISA kit; EC 3.5.3.1 ELISA kit; Liver-type arginase ELISA kit; Type I arginase ELISA kit

Abbreviation

ARG1

Uniprot No.

Q61176

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.