Mouse Bone morphogenetic protein 4,BMP-4 ELISA Kit
Product Details
| Target Name |
bone morphogenetic protein 4 |
| Alternative Names |
Bmp4 ELISA kit; Bmp-4 ELISA kit; Dvr-4Bone morphogenetic protein 4 ELISA kit; BMP-4 ELISA kit; Bone morphogenetic protein 2B ELISA kit; BMP-2B ELISA kit |
| Abbreviation |
BMP4 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 pg/mL-40 pg/mL |
| Sensitivity |
0.156 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-112 |
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| 1:4 |
Average % |
85 |
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| Range % |
80-110 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of mouse BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
92 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.657 |
2.677 |
2.667 |
2.509 |
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| 20 |
2.212 |
2.302 |
2.257 |
2.099 |
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| 10 |
1.583 |
1.683 |
1.633 |
1.475 |
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| 5 |
1.042 |
1.082 |
1.062 |
0.904 |
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| 2.5 |
0.597 |
0.622 |
0.610 |
0.452 |
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| 1.25 |
0.384 |
0.401 |
0.393 |
0.235 |
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| 0.625 |
0.288 |
0.302 |
0.295 |
0.137 |
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| 0 |
0.162 |
0.154 |
0.158 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
