This Mouse C-Reactive Protein (CRP) ELISA Kit is used to quantitatively determine mouse CRP concentrations in serum, plasma, cell culture supernates, or tissue homogenates. CRP is an acute inflammatory protein that exhibits significantly elevated [removed]1,000-fold) at sites of infection or inflammation. It has been traditionally used as a marker of infection and cardiovascular events. Increasing evidence shows that CRP plays important role in inflammatory processes and host defense to infection including the complement pathway, apoptosis, phagocytosis, NO release, and the production of cytokines, particularly IL-6 and TNF-α.

This kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse CRP in the sample. Antibody specific for CRP has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated CRP antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and color develops into blue. The color changes from blue to yellow after the addition of stop solution into the wells. The intensity of the color is in proportion to the amount of CRP bound in the initial step. The absorbance (OD value) was determined by the microplate reader at the wavelength of 450nm, and the concentration of mouse NT-proBNP was calculated through the standard curve. Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See the instructions for more information on validation