Mouse Caspase 3,Casp-3 ELISA Kit
Product Details
| Target Name |
caspase 3, apoptosis-related cysteine peptidase |
| Alternative Names |
Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit |
| Abbreviation |
CASP3 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse caspase 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:2 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:4 |
Average % |
103 |
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| Range % |
98-107 |
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| 1:8 |
Average % |
90 |
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| Range % |
84-95 |
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| Recovery |
| The recovery of mouse caspase 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-105 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.637 |
2.552 |
2.595 |
2.437 |
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| 10 |
2.175 |
2.106 |
2.141 |
1.983 |
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| 5 |
1.464 |
1.524 |
1.494 |
1.336 |
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| 2.5 |
0.832 |
0.829 |
0.831 |
0.673 |
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| 1.25 |
0.543 |
0.524 |
0.534 |
0.376 |
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| 0.625 |
0.366 |
0.339 |
0.353 |
0.195 |
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| 0.312 |
0.269 |
0.285 |
0.277 |
0.119 |
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| 0 |
0.159 |
0.157 |
0.158 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
