Mouse Corticosterone,CORT ELISA Kit
Product Details
| Description |
The Mouse Corticosterone (CORT) ELISA Kit is specifically designed to standardize the detection of mouse CORT in multiple biological solutions, including serum, plasma, and tissue homogenates. This kit employs the Competitive-ELISA mechanism to quantitatively measure the concentration of CORT in the sample. The microtiter plate has been pre-coated with the goat anti-rabbit CORT antibody. Standards or samples are pipetted into the microtiter plate with the anti-mouse CORT antibody and HRP-conjugate. A competitive inhibition reaction is launched between CORT (standards or samples) and HRP-conjugated CORT with the anti-mouse CORT antibody. And these Ag-Ab complexes are captured by the antibody immobilized on the plate. Following a wash to remove any unbound reagent, substrate A and B solutions are added to the wells to elicit a chromogenic reaction. The color development is terminated after adding the stop solution, and the solution color changes from blue to yellow. The color intensity
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| Target Name |
Corticosterone,CORT |
| Alternative Names |
N/A |
| Abbreviation |
CORT |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.1 ng/mL-20 ng/mL |
| Sensitivity |
0.05 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:4 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-101 |
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| Recovery |
| The recovery of mouse CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
93-104 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 20 |
0.128 |
0.122 |
0.125 |
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| 5 |
0.238 |
0.229 |
0.234 |
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| 1.6 |
0.424 |
0.459 |
0.442 |
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| 0.4 |
0.921 |
0.959 |
0.940 |
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| 0.1 |
1.524 |
1.619 |
1.572 |
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| 0 |
2.354 |
2.219 |
2.287 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
- Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
- HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
- Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
- Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
- Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
- An Instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
