| Description |
The ELISA kit CSB-E15061m is applied to quantitatively detect the content of the CRAMP in samples of serum, plasma, cell culture supernates, or tissue homogenates. The detection mechanism of this mouse CRAMP ELISA kit is based on the sandwich assay. The standards or samples are added to the microtiter plate wells pre-coated with CRAMP specific antibody. Biotin-labeled CRAMP antibody and HRP-avidin are successively pipped into the wells, forming the immune complex. The solution develops color after the addition of TMB substrate. The content of CRAMP can be quantitatively determined by measuring the optical density of color with a microplate reader. This ELISA kit has been verified with high sensitivity (0.39 pg/mL), excellent specificity, a precision of less than 10%, good correlation and linearity, and high recovery. The mouse CRAMP is the sole identified murine cathelicidin and the ortholog of the human LL-37. CRAMP forms a positively charged amphipathic α-helical structure and posses... Read more |
| Target Name |
Cathelicidin antimicrobial peptide |
| Alternative Names |
Camp ELISA Kit; Cnlp ELISA Kit; CrampCathelicidin antimicrobial peptide ELISA Kit; Cathelin-like protein ELISA Kit; CLP) [Cleaved into: Cathelin-related antimicrobial peptide ELISA Kit; Cramp)] ELISA Kit |
| Abbreviation |
CAMP |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse cramp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:200 |
Average % |
95 |
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| Range % |
91-98 |
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| 1:400 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:800 |
Average % |
93 |
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| Range % |
87-99 |
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| Recovery |
| The recovery of mouse cramp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
82-94 |
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| EDTA plasma (n=4) |
102 |
94-109 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.300 |
2.200 |
2.250 |
2.154 |
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| 50 |
1.505 |
1.405 |
1.455 |
1.359 |
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| 25 |
0.822 |
0.802 |
0.812 |
0.716 |
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| 12.5 |
0.445 |
0.435 |
0.440 |
0.344 |
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| 6.25 |
0.314 |
0.304 |
0.309 |
0.213 |
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| 3.12 |
0.211 |
0.201 |
0.206 |
0.110 |
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| 1.56 |
0.149 |
0.147 |
0.148 |
0.052 |
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| 0 |
0.097 |
0.095 |
0.096 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse CRAMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled CRAMP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
