Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit
Product Details
| Target Name |
Creatine Kinase MB isoenzyme,CK-MB |
| Abbreviation |
CK-MB |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
1.25 mU/mL-80 mU/mL |
| Sensitivity |
0.312 mU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
80-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
86-105 |
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| 1:4 |
Average % |
91 |
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| Range % |
82-100 |
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| 1:8 |
Average % |
93 |
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| Range % |
82-101 |
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| Recovery |
| The recovery of mouse CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
94-105 |
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| EDTA plasma (n=4) |
95 |
89-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.912 |
2.787 |
2.850 |
2.684 |
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| 40 |
2.386 |
2.374 |
2.380 |
2.214 |
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| 20 |
1.617 |
1.664 |
1.641 |
1.475 |
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| 10 |
0.846 |
0.895 |
0.871 |
0.705 |
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| 5 |
0.553 |
0.523 |
0.538 |
0.372 |
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| 2.5 |
0.318 |
0.336 |
0.327 |
0.161 |
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| 1.25 |
0.246 |
0.252 |
0.249 |
0.083 |
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| 0 |
0.164 |
0.168 |
0.166 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
