Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit
Product Details
| Target Name |
Creatine Kinase MM isoenzyme,CK-MM |
| Abbreviation |
CK-MM |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma |
| Detection Range |
0.156 mU/mL-10 mU/mL |
| Sensitivity |
0.039 mU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
87 |
|
| Range % |
83-98 |
|
| 1:2 |
Average % |
98 |
|
| Range % |
92-103 |
|
| 1:4 |
Average % |
104 |
|
| Range % |
98-108 |
|
| 1:8 |
Average % |
93 |
|
| Range % |
87-99 |
|
|
| Recovery |
| The recovery of mouse CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
85-99 |
|
| EDTA plasma (n=4) |
100 |
95-105 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.420 |
2.566 |
2.493 |
2.343 |
|
| 5 |
1.708 |
1.855 |
1.782 |
1.632 |
|
| 2.5 |
0.970 |
1.002 |
0.986 |
0.836 |
|
| 1.25 |
0.556 |
0.601 |
0.579 |
0.429 |
|
| 0.625 |
0.359 |
0.369 |
0.364 |
0.214 |
|
| 0.312 |
0.270 |
0.278 |
0.274 |
0.124 |
|
| 0.156 |
0.197 |
0.194 |
0.196 |
0.046 |
|
| 0 |
0.155 |
0.145 |
0.150 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
