Mouse Cytochrome-C,Cyt-C ELISA Kit
Product Details
| Target Name |
cytochrome c, somatic |
| Alternative Names |
Cycs ELISA Kit; Cytochrome c ELISA Kit; somatic ELISA Kit |
| Abbreviation |
CYCS |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
34.3 pg/mL-2200 pg/mL |
| Sensitivity |
8.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-97 |
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| 1:2 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-101 |
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| Recovery |
| The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
101 |
95-105 |
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| EDTA plasma (n=4) |
87 |
83-91 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2200 |
2.352 |
2.512 |
2.432 |
2.240 |
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| 1100 |
1.615 |
1.578 |
1.597 |
1.405 |
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| 550 |
1.073 |
1.023 |
1.048 |
0.856 |
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| 275 |
0.639 |
0.681 |
0.660 |
0.468 |
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| 137.5 |
0.461 |
0.489 |
0.475 |
0.283 |
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| 68.75 |
0.387 |
0.374 |
0.381 |
0.189 |
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| 34.3 |
0.318 |
0.305 |
0.312 |
0.120 |
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| 0 |
0.194 |
0.189 |
0.192 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
