Mouse E-Selectin ELISA kit
Product Details
| Target Name |
selectin E |
| Alternative Names |
Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit |
| Abbreviation |
SELE |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse E-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
90-96 |
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| 1:2 |
Average % |
85 |
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| Range % |
81-90 |
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| 1:4 |
Average % |
104 |
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| Range % |
100-108 |
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| 1:8 |
Average % |
86 |
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| Range % |
82-90 |
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| Recovery |
| The recovery of mouse E-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
102 |
98-106 |
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| EDTA plasma (n=4) |
89 |
85-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.076 |
2.131 |
2.104 |
1.907 |
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| 2500 |
1.671 |
1.638 |
1.655 |
1.458 |
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| 1250 |
1.168 |
1.201 |
1.185 |
0.988 |
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| 625 |
0.829 |
0.831 |
0.830 |
0.633 |
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| 312 |
0.505 |
0.511 |
0.508 |
0.311 |
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| 156 |
0.365 |
0.372 |
0.369 |
0.172 |
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| 78 |
0.285 |
0.289 |
0.287 |
0.090 |
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| 0 |
0.195 |
0.199 |
0.197 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
