Mouse Endothelin 1,ET-1 ELISA Kit
Product Details
| Target Name |
endothelin 1 |
| Alternative Names |
Edn1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit |
| Abbreviation |
EDN1 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
83-94 |
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| 1:2 |
Average % |
93 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
88-99 |
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| Recovery |
| The recovery of mouse ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-102 |
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| EDTA plasma (n=4) |
98 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.729 |
2.612 |
2.671 |
2.524 |
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| 500 |
1.938 |
1.876 |
1.907 |
1.760 |
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| 250 |
1.228 |
1.204 |
1.216 |
1.069 |
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| 125 |
0.816 |
0.759 |
0.788 |
0.641 |
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| 62.5 |
0.435 |
0.407 |
0.421 |
0.274 |
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| 31.2 |
0.299 |
0.275 |
0.287 |
0.140 |
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| 15.6 |
0.241 |
0.223 |
0.232 |
0.085 |
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| 0 |
0.151 |
0.143 |
0.147 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
