The Mouse estrogen (E) ELISA Kit is specifically designed to standardize the detection of mouse estrogen in serum and plasma. This kit employs the Competitive-ELISA mechanism to quantitatively measure the concentration of estrogen. The goat-anti-rabbit estrogen antibody has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells with anti-mouse estrogen antibody and HRP-conjugated estrogen. A competitive inhibition reaction is launched between estrogen (standards or samples) and HRP-conjugated estrogen with the anti-mouse estrogen antibody. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells. HRP-TMB interaction elicits a chromogenic reaction. The color development is terminated, and the solution color changes from blue to yellow. The color develops in opposite to the amount of estrogen in the sample. This kit has been professionally verified with reliable quality, high sensitivity, excellent specificity, good linearity, precision less than 15%, and consistency between batches. See more details on the product instructions.

Estrogen is a female reproductive organ-associated sex hormone responsible for the development of female sexual characteristics. As a steroid hormone, estrogen can diffuse into cells freely and translocate into the nucleus, where it binds to estrogen receptors, inducing gene expression that ultimately triggers a physical response. Estrogen levels in the body are regulated by the negative feedback effect of estrogen on the hypothalamus and pituitary gland. Additionally, estrogen also plays important roles in the development, maturation, and aging of extragonadal tissues such as bone, muscle, and connective tissues.