Mouse free testosterone (F-TESTO) ELISA kit
Product Details
| Target Name |
free testosterone |
| Abbreviation |
F-TESTO |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma |
| Detection Range |
5 pg/mL-1000 pg/mL |
| Sensitivity |
2.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Epigenetics and Nuclear Signaling |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
94 |
|
|
| Range % |
86-102 |
|
|
| 1:2 |
Average % |
95 |
|
|
| Range % |
91-99 |
|
|
| 1:4 |
Average % |
92 |
|
|
| Range % |
89-102 |
|
|
| 1:8 |
Average % |
94 |
|
|
| Range % |
82-100 |
|
|
|
| Recovery |
| The recovery of mouse F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
98 |
92-110 |
|
|
| EDTA plasma (n=4) |
97 |
90-107 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
|
| 0 |
2.675 |
2.593 |
2.634 |
|
|
|
| 5 |
1.456 |
1.397 |
1.427 |
|
|
|
| 20 |
0.958 |
1.011 |
0.985 |
|
|
|
| 80 |
0.496 |
0.502 |
0.499 |
|
|
|
| 250 |
0.347 |
0.332 |
0.340 |
|
|
|
| 1000 |
0.163 |
0.158 |
0.161 |
|
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
