Mouse glutathione peroxidase(GSH-Px)ELISA Kit
Product Details
| Target Name |
glutathione peroxidase(GSH-Px) |
| Alternative Names |
Gpx1 ELISA Kit; Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit; Selenium-dependent glutathione peroxidase 1 ELISA Kit |
| Abbreviation |
GSH-Px |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 U/mL-2000 U/mL |
| Sensitivity |
7.8 U/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
97 |
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| Range % |
91-102 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of mouse GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
93-102 |
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| EDTA plasma (n=4) |
88 |
82-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| U/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.324 |
2.401 |
2.363 |
2.275 |
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| 1000 |
1.602 |
1.699 |
1.651 |
1.563 |
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| 500 |
1.056 |
1.039 |
1.048 |
0.960 |
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| 250 |
0.657 |
0.665 |
0.661 |
0.573 |
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| 125 |
0.431 |
0.446 |
0.439 |
0.351 |
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| 62.5 |
0.274 |
0.285 |
0.280 |
0.192 |
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| 31.25 |
0.158 |
0.162 |
0.160 |
0.072 |
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| 0 |
0.087 |
0.088 |
0.088 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
