Mouse Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit
Product Details
| Target Name |
colony stimulating factor 2 (granulocyte-macrophage) |
| Alternative Names |
Csf2 ELISA Kit; Csfgm ELISA Kit; Granulocyte-macrophage colony-stimulating factor ELISA Kit; GM-CSF ELISA Kit; Colony-stimulating factor ELISA Kit; CSF ELISA Kit |
| Abbreviation |
CSF2 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
88-100 |
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| 1:2 |
Average % |
94 |
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| Range % |
84-104 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
90 |
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| Range % |
83-99 |
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| Recovery |
| The recovery of mouse GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
83-103 |
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| EDTA plasma (n=4) |
96 |
89-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.762 |
2.856 |
2.809 |
2.633 |
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| 500 |
2.190 |
2.223 |
2.207 |
2.031 |
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| 250 |
1.411 |
1.435 |
1.423 |
1.247 |
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| 125 |
0.845 |
0.856 |
0.851 |
0.675 |
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| 62.5 |
0.556 |
0.561 |
0.559 |
0.383 |
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| 31.2 |
0.378 |
0.382 |
0.380 |
0.204 |
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| 15.6 |
0.254 |
0.269 |
0.262 |
0.086 |
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| 0 |
0.175 |
0.176 |
0.176 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
