Mouse granzyme B (GZMB) ELISA Kit
Product Details
| Target Name |
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
| Alternative Names |
Gzmb ELISA kit; Ctla-1 ELISA kit; Ctla1Granzyme B(G,H ELISA kit; EC 3.4.21.79 ELISA kit; CTLA-1 ELISA kit; Cytotoxic cell protease 1 ELISA kit; CCP1 ELISA kit; Fragmentin-2 ELISA kit |
| Abbreviation |
GZMB |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.12 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gzms-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
102 |
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| Range % |
95-106 |
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| 1:2 |
Average % |
86 |
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| Range % |
82-92 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of mouse Gzms-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
103 |
96-107 |
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| EDTA plasma (n=4) |
93 |
88-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.076 |
2.143 |
2.110 |
1.985 |
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| 400 |
1.723 |
1.812 |
1.768 |
1.643 |
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| 200 |
1.262 |
1.292 |
1.277 |
1.152 |
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| 100 |
0.783 |
0.796 |
0.790 |
0.665 |
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| 50 |
0.402 |
0.415 |
0.409 |
0.284 |
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| 25 |
0.276 |
0.280 |
0.278 |
0.153 |
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| 12.5 |
0.191 |
0.195 |
0.193 |
0.068 |
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| 0 |
0.124 |
0.126 |
0.125 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
