Mouse Heat Shock Protein 90,Hsp-90 ELISA Kit
Product Details
| Target Name |
heat shock protein 90kDa alpha (cytosolic), class A member 1 |
| Alternative Names |
Hsp90aa1 ELISA Kit; Hsp86 ELISA Kit; Hsp86-1 ELISA Kit; HspcaHeat shock protein HSP 90-alpha ELISA Kit; Heat shock 86 kDa ELISA Kit; HSP 86 ELISA Kit; HSP86 ELISA Kit; Tumor-specific transplantation 86 kDa antigen ELISA Kit; TSTA ELISA Kit |
| Abbreviation |
HSP90AA1 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hsp-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:5 |
Average % |
93 |
|
| Range % |
81-101 |
|
| 1:10 |
Average % |
95 |
|
| Range % |
86-104 |
|
| 1:20 |
Average % |
95 |
|
| Range % |
82-105 |
|
| 1:40 |
Average % |
93 |
|
| Range % |
82-101 |
|
|
| Recovery |
| The recovery of mouse Hsp-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-105 |
|
| EDTA plasma (n=4) |
95 |
89-110 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.773 |
2.787 |
2.780 |
2.665 |
|
| 5 |
1.842 |
1.740 |
1.791 |
1.676 |
|
| 2.5 |
1.105 |
1.164 |
1.135 |
1.020 |
|
| 1.25 |
0.668 |
0.673 |
0.671 |
0.556 |
|
| 0.625 |
0.398 |
0.403 |
0.401 |
0.286 |
|
| 0.312 |
0.282 |
0.266 |
0.274 |
0.159 |
|
| 0.156 |
0.159 |
0.152 |
0.156 |
0.041 |
|
| 0 |
0.112 |
0.118 |
0.115 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
