Mouse High density lipoprotein cholesterol, HDL-C ELISA kit
Product Details
| Target Name |
High density lipoprotein cholesterol, HDL-C |
| Abbreviation |
HDL-C |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 ng/mL-500 ng/mL |
| Sensitivity |
1.95 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
88 |
|
| Range % |
84-92 |
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| 1:2000 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:4000 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:8000 |
Average % |
92 |
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| Range % |
88-96 |
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| Recovery |
| The recovery of mouse HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-102 |
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| EDTA plasma (n=4) |
97 |
91-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.509 |
2.598 |
2.554 |
2.440 |
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| 250 |
2.045 |
2.068 |
2.057 |
1.943 |
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| 125 |
1.401 |
1.457 |
1.429 |
1.315 |
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| 62.5 |
0.977 |
0.999 |
0.988 |
0.874 |
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| 31.2 |
0.572 |
0.598 |
0.585 |
0.471 |
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| 15.6 |
0.315 |
0.338 |
0.327 |
0.213 |
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| 7.8 |
0.256 |
0.267 |
0.262 |
0.148 |
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| 0 |
0.113 |
0.115 |
0.114 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
