Mouse High mobility group protein B1,HMGB-1 ELISA Kit
Product Details
| Target Name |
high-mobility group box 1 |
| Alternative Names |
Hmgb1 ELISA Kit; Hmg-1 ELISA Kit; Hmg1High mobility group protein B1 ELISA Kit; High mobility group protein 1 ELISA Kit; HMG-1 ELISA Kit |
| Abbreviation |
HMGB1 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Epigenetics and Nuclear Signaling |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
92 |
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| Range % |
80-98 |
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| 1:200 |
Average % |
85 |
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| Range % |
81-96 |
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| 1:400 |
Average % |
97 |
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| Range % |
90-104 |
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| 1:800 |
Average % |
95 |
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| Range % |
82-98 |
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| Recovery |
| The recovery of mouse HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
95-100 |
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| EDTA plasma (n=4) |
95 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.756 |
2.712 |
2.734 |
2.565 |
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| 500 |
2.223 |
2.251 |
2.237 |
2.068 |
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| 250 |
1.547 |
1.572 |
1.560 |
1.391 |
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| 125 |
1.020 |
0.986 |
1.003 |
0.834 |
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| 62.5 |
0.598 |
0.621 |
0.610 |
0.441 |
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| 31.2 |
0.452 |
0.432 |
0.442 |
0.273 |
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| 15.6 |
0.287 |
0.275 |
0.281 |
0.112 |
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| 0 |
0.173 |
0.164 |
0.169 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
