Mouse Insulin-like growth factor 2,IGF-2 ELISA Kit
Product Details
| Target Name |
insulin-like growth factor 2 (somatomedin A) |
| Alternative Names |
Igf2 ELISA Kit; Igf-2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Multiplication-stimulating polypeptide) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit |
| Abbreviation |
IGF2 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
88 |
|
| Range % |
84-97 |
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| 1:200 |
Average % |
96 |
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| Range % |
91-102 |
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| 1:400 |
Average % |
84 |
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| Range % |
81-88 |
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| 1:800 |
Average % |
92 |
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| Range % |
88-97 |
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| Recovery |
| The recovery of mouse IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
95-106 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 2000 |
2.637 |
2.774 |
2.706 |
2.554 |
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| 1000 |
2.343 |
2.451 |
2.397 |
2.245 |
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| 500 |
1.837 |
1.935 |
1.886 |
1.734 |
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| 250 |
1.103 |
1.190 |
1.147 |
0.995 |
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| 125 |
0.610 |
0.632 |
0.621 |
0.469 |
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| 62.5 |
0.374 |
0.355 |
0.365 |
0.213 |
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| 31.25 |
0.244 |
0.248 |
0.246 |
0.094 |
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| 0 |
0.150 |
0.153 |
0.152 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
