Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit
Product Details
| Target Name |
insulin-like growth factor binding protein 4 |
| Alternative Names |
Igfbp4 ELISA Kit; Igfbp-4 ELISA Kit; Insulin-like growth factor-binding protein 4 ELISA Kit; IBP-4 ELISA Kit; IGF-binding protein 4 ELISA Kit; IGFBP-4 ELISA Kit |
| Abbreviation |
IGFBP4 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:8 |
Average % |
105 |
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| Range % |
100-112 |
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| Recovery |
| The recovery of mouse IGFBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
88 |
84-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.384 |
2.495 |
2.440 |
2.290 |
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| 25 |
2.041 |
2.103 |
2.072 |
1.922 |
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| 12.5 |
1.605 |
1.569 |
1.587 |
1.437 |
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| 6.25 |
1.002 |
0.981 |
0.992 |
0.842 |
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| 3.12 |
0.519 |
0.538 |
0.529 |
0.379 |
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| 1.56 |
0.339 |
0.352 |
0.346 |
0.196 |
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| 0.78 |
0.263 |
0.274 |
0.269 |
0.119 |
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| 0 |
0.152 |
0.147 |
0.150 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
