Mouse intercellular adhesion molecule 1,ICAM-1 ELISA KIT
Product Details
| Target Name |
intercellular adhesion molecule 1 |
| Alternative Names |
Icam1 ELISA Kit; Icam-1 ELISA Kit; Intercellular adhesion molecule 1 ELISA Kit; ICAM-1 ELISA Kit; MALA-2 ELISA Kit; MyD10 ELISA Kit; CD antigen CD54 ELISA Kit |
| Abbreviation |
ICAM1 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78 ng/mL-5000 ng/mL |
| Sensitivity |
19.5 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-92 |
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| 1:4 |
Average % |
96 |
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| Range % |
85-105 |
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| 1:8 |
Average % |
93 |
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| Range % |
83-98 |
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| Recovery |
| The recovery of mouse ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
82-101 |
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| EDTA plasma (n=4) |
94 |
86-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.555 |
2.518 |
2.537 |
2.390 |
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| 2500 |
2.047 |
2.105 |
2.076 |
1.929 |
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| 1250 |
1.531 |
1.552 |
1.542 |
1.395 |
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| 625 |
0.812 |
0.803 |
0.808 |
0.661 |
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| 312 |
0.516 |
0.524 |
0.520 |
0.373 |
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| 156 |
0.312 |
0.321 |
0.317 |
0.170 |
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| 78 |
0.229 |
0.225 |
0.227 |
0.080 |
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| 0 |
0.145 |
0.149 |
0.147 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
