Mouse interferon-inducible protein 10,IP-10 ELISA Kit
Product Details
| Target Name |
chemokine (C-X-C motif) ligand 10 |
| Alternative Names |
Cxcl10 ELISA kit; Crg2 ELISA kit; Ifi10 ELISA kit; Inp10 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; C7 ELISA kit; Interferon-gamma induced protein CRG-2 ELISA kit; Small-inducible cytokine B10 ELISA kit |
| Abbreviation |
CXCL10 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
86-93 |
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| 1:2 |
Average % |
101 |
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| Range % |
94-105 |
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| 1:4 |
Average % |
92 |
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| Range % |
85-96 |
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| 1:8 |
Average % |
96 |
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| Range % |
91-101 |
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| Recovery |
| The recovery of mouse IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
86-96 |
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| EDTA plasma (n=4) |
95 |
87-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.332 |
2.214 |
2.273 |
2.122 |
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| 100 |
1.645 |
1.621 |
1.633 |
1.482 |
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| 50 |
1.125 |
1.222 |
1.174 |
1.023 |
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| 25 |
0.685 |
0.677 |
0.681 |
0.530 |
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| 12.5 |
0.484 |
0.465 |
0.475 |
0.324 |
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| 6.25 |
0.322 |
0.311 |
0.317 |
0.166 |
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| 3.12 |
0.213 |
0.229 |
0.221 |
0.070 |
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| 0 |
0.150 |
0.152 |
0.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
